Auteur Topic: Zijn patiŽnten met colitis ulcerosa gebaat bij een tarwevrij dieet?  (gelezen 432 keer)

tine

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Het zou best eens zo kunnen zijn dat patiŽnten met colitis ulcerosa, een chronische ontstekingsziekte van de dikke darm, gebaat zijn bij een eetpatroon zonder tarwe. Duitse onderzoekers tonen aan dat muizen die beschadigde darmen hebben, verder achteruitgaan als ze tarwe eten. De  ontstekingen in hun darmen worden ernstiger. Ook de populatie bacteriŽn in hun darmen is niet meer in balans. De oorzaak lijkt te liggen bij ATIs, eiwitten in tarwe die de plant beschermen tegen plagen zoals wolluis en vraat van dieren. Of je deze uitkomsten zomaar kunt vertalen naar mensen is niet bekend. Daarvoor is eerst meer onderzoek nodig.   

Gastroenterology. 2020 Jul;159(1):257-272.e17.
 doi: 10.1053/j.gastro.2020.03.064. Epub 2020 Apr 3.
Wheat Consumption Aggravates Colitis in Mice via Amylase Trypsin Inhibitor-mediated Dysbiosis
Geethanjali Pickert 1, Stefan Wirtz 2, Johannes Matzner 1, Muhammad Ashfaq-Khan 1, Rosario Heck 1, Sebastian Rosigkeit 1, Dorothe Thies 1, Rambabu Surabattula 1, Dirk Ehmann 3, Jan Wehkamp 3, Misbah Aslam 1, Guiwei He 2, Andreas Weigert 4, Friedrich Foerster 5, Luisa Klotz 6, Julia-Stefanie Frick 7, Christoph Becker 2, Ernesto Bockamp 1, Detlef Schuppan 8

Background & aims:

Wheat has become the world's major staple and its consumption correlates with prevalence of noncommunicable disorders such as inflammatory bowel diseases. Amylase trypsin inhibitors (ATIs), a component of wheat, activate the intestine's innate immune response via toll-like receptor 4 (TLR4). We investigated the effects of wheat and ATIs on severity of colitis and fecal microbiota in mice.

Methods:

C57BL/6 wild-type and Tlr4-/- mice were fed wheat- or ATI-containing diets or a wheat-free (control) diet and then given dextran sodium sulfate to induce colitis; we also studied Il10-/- mice, which develop spontaneous colitis. Changes in fecal bacteria were assessed by taxa-specific quantitative polymerase chain reaction and 16S ribosomal RNA metagenomic sequencing. Feces were collected from mice on wheat-containing, ATI-containing, control diets and transplanted to intestines of mice with and without colitis on control or on ATI-containing diets. Intestinal tissues were collected and analyzed by histology, immunohistochemistry, and flow cytometry. Bacteria with reported immunomodulatory effects were incubated with ATIs and analyzed in radial diffusion assays.

Results:

The wheat- or ATI-containing diets equally increased inflammation in intestinal tissues of C57BL/6 mice with colitis, compared with mice on control diets. The ATI-containing diet promoted expansion of taxa associated with development of colitis comparable to the wheat-containing diet. ATIs inhibited proliferation of specific human commensal bacteria in radial diffusion assays. Transplantation of microbiota from feces of mice fed the wheat- or ATI-containing diets to intestines of mice on control diets increased the severity of colitis in these mice. The ATI-containing diet did not increase the severity of colitis in Tlr4-/- mice.
Conclusions: Consumption of wheat or wheat ATIs increases intestinal inflammation in mice with colitis, via TLR4, and alters their fecal microbiota. Wheat-based, ATI-containing diets therefore activate TLR4 signaling and promote intestinal dysbiosis.

PMID: 32251667
DOI: 10.1053/j.gastro.2020.03.064


Mijn zoon (20) en ik eten allebei glutenvrij. Wij zijn extreem gevoelig voor sporen van gluten (via besmetting, tarwe-derivaten en hulpstoffen). Ik ben wetenschapsredacteur voor het Glutenvrij Magazine van de NCV.